Analysis of Glycans and Glycoproteins by Mass Spectrometry

Analysis of Glycans and Glycoproteins by Mass Spectrometry

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Glycosylation is among the important post-translational modifications that occurs in proteins. In particular the heterogeneity of the glycan moiety can affect a range of physio-chemical properties of a glycoprotein. Thus the increasing interest in glycan function has created a need for sensitive detection and analysis.Preparation of derivatives a€“ purification by HPLC Preparation of derivatives for HPLC was done using the same synthetic procedure described above, using ... The derivatized oligosaccharides were separated and desalted by RP-HPLC using Shimadzu model LC-10ATvp. ... HPLC fractions were collected manually.

Title:Analysis of Glycans and Glycoproteins by Mass Spectrometry
Publisher:ProQuest - 2007

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